Environmental variables
Environment-Genotype association is considered to detect patterns of adaptive genetic variation
GWAS
Genome-Wide association studies focus on SNPs variants associated with specific variables or traits.
(e.g. chemotype)
Chemotype
Chemotype-Genotype association is considered to detect patterns of adaptive genetic variation.
Transcriptomics
Using comparative gene expression profiling candidate genes are selected. Generated markers will be employed to detect variation in genes relevant to adaptation
Genotyping
Detect loci under selection
The “best” 500 genes are selected for genotyping (SNPs). 2000 individuals from ~ 100 populations are genotyped.
HPLC phenotyping
Chemotype patterns from different populations collected along the environmental gradients are compared. Presence of adaptive chemotypic variation is verified.
Is chemical pattern genetically fixed?
Heritability test comparing parent (f) chemotype with offspring is conducted.
Why common garden?
Herbivory affects secondary metabolites patterns(and vice-versa). Thus, is necessary to minimise environmental effect by growing samples in a „common garden“
Sample size
Seeds and leaves from ~2000 individuals/ ~ 100 pop. Collected throughout Europe in 2018, 2019 amd 2020.
Environmental gradients
Sampling designed to cover the main environmental gradients (latitude, longitude, altitude, climate, etc. )
STEP BY STEP
